Inertsil ods hplc columns pdf

Ymctriart c18 is a hybrid silica based ods column emphasizing durability, low operating pressure, and excellent performance. Gl sciences hplc columns can tolerate up to pressures shown below. Inertsil c84 is an octyl group c8 bonded column providing the same separation pattern and extreme inertness to any type of compounds just like inertsil ods4, which enables rapid analysis of highly hydrophobic compounds delivering symmetric peaks and easy method transfer from. Hypersil ods2 c18 columns thermo fisher scientific. Hplc column selection a comparison of reversedphase columns based on the widely accepted work and data of drs. Inertsil sulfa c18 inertsil sulfa c18 3 and 5m is designed for the analysis of sulpha drugs. Inertsil ods3v is a highly efficient, validated hplc column. We use cookies to improve your experience and our website service. Specifically qualified hplc columns for glpgmp compliance validation inertsil ods3v offers all of the outstanding chromatographic benefits of inertsil. We are manufacturer, exporter, wholesaler, supplier, distributor and importer of hplc columns, vials and filter paper. A comparison of alltech c18 and c8 columns alltech has developed a method for choosing c18 and c8 columns based on peak capacities and column selectivity of polar and nonpolar compounds. The same high performance characteristics found in inertsil ods3 have been incorporated in the manufacture of inertsil ods3v.

Hl recognizes even slight steric differences achieving complete baseline separation while other conventional c18 column fails. A 100% water mobile phase hplcpda analysis of meamine and. Inertsil ods3 analytical columns products gl sciences. Inertsil ods inertsil ods series 1,2,3 isco c18 jsphere odsh80 jsphere odsl80 jsphere odsm80 jupiter c18 kinetex c18 kromasil c18 lichrospher pah lichrosorb rp18 lichrospher 100 rp18 lichrospher rp18e luna c18 luna c182 diazem c18 60a series 1,2,3 diazem c18 series ec diazem c18 dualzone discovery c18 discovery hs c18. Inertsil 4 series columns are the most recent addition to the inertsil line and the most inert columns available. The result is a series of columns which provide excellent peak shapes using simple eluents. Conditions chosen based on simplicity of mobile phase for nonionic. Inertsil hplc packings are subject to a rigorous array of qc tests in a iso9001 compliant facility, with special emphasis on reagent purity, raw material traceability, and consistency in raw materials and finished.

The offered products are widely demanded for their features like safe usage, easy to rinse and reasonable prices. Proven as an effective analytical tool, the classical hypersil phases are well established and referenced in many hplc methods worldwide. The typical particle size of traditional hplc columns is 5m, but in the past, 3. Ods 80a was originally introduced to provide a higher surface area alternative to ods 2, but with the same inertness and basedeactivated properties. Inertsil odsep c18 embedded polar functional groups is compatible with 100% aqueous eluents. In addition, the introduction of higher surface area silica. Luna c18 ace superc18 ymcpack odsam symmetry c18 kromasil c18 lichrosorb rp18 nucleodur c18 nucleosil c18 inertsil ods2 c18 acclaim c18. Inertsiltm ods4v 3 m, 5 m manufacturers validation certificate sheet inertsil ods4 columns have proven superior worldwide for analysis of strong pharmaceutical bases, acids, chelating compounds, and zwitterions. Inertsil odssprint inertsil peptide c18 the inertsil peptide c18 column is speci cally suited to the analysis of peptide digests. All standard geometries available most from stock priced competitively gl sciences inertsil hplc columns offer a significant advantage in the analysis of basic molecules, amines and accidic compunds.

Inertsil ods4 column name functional group inertsustain c18 inherits the advantages of all the current inertsil hplc columns, but now can be used for wide ph analysis with consistent performance. Ymctriart c18 has been developed and is manufactured using stateoftheart technologies that define materials, granulation. Hypersil ods 5 120 170 10 yes no inertsil ods 5 100 350 14 yes no inertsil ods3 5 100 450 15 yes yes inertsil ods2 5 150 320 18. Graduate school of human life science, osaka city university, osaka, japan. Thermo scientific hypersil ods2 c18 hplc columns are an alternative to older column brands commonly referenced in validated methods. Inertsil ods columns are general purpose, reversed phase c18 columns. Shop online for a wide selection of thermo scientific hypersil ods c18 columns use as a general purpose c18 phase for acidic and neutral analytes. Inertsil ods4 minimizes the dewetting phenomenon while maintaining the same retention characteristics to that of common ods columns. Their low cost makes them the most widely used columns for hplc. Specifically qualified hplc columns for glpgmp compliance. Classical phases tm hypersil thermo electron corporation. Inertsil ods 3 users can, therefore, readily change to the new, validated inertsil ods 3v columns with complete confidence. From the first generation inertsil ods to the latest inertsustain series, the inertsil series columns are widely used around the world ensuring column quality and performance anywhere in the world. Inertsil ods4 and c84 columns also show excellent stability during the analysis of strong metal.

Typically chromatographers must choose hplc columns by comparing the surface area and carbon load data. Inertsil ods 3 is still gl sciences most popular phase and continues to be used world widely and reliably for long established methods in pharmaceutical, and contract research labs. The same high performance characteristics found in inertsil ods 3 have been incorporated in the manufacture of inertsil ods 3v. Inertsil inods3150dea 0 gbp inods3150d 5547593 5547596 502001900 5547594 inods3250d 502001921 5547588 5547595 5547585 5547587 502005044 5547586 inods2150a. Inertsil c4 inertsil wp300 c4 microsorb c4 bondesil silica inertsil econo prepsil generik silica generik c8amino protec c8 chromegabond c2e gpc2 nucleosil c2 l31 l32 l33 l34 l35 l36 usp listing of hplc columns l packingbrand name brand name l37 l38 l39 l40 l41 l43 l44 l51 l48 l49 l52 l53 l54 l55 l56 l57 l58 l59 l60 l61 packing having the. However, a variety of problems may arise while using these columns. H 1 h 2 is the difference between the hydrophobicity parameters of columns 1 and 2 s 1 s 2 is the difference between the steric parameters of columns 1 and 2 a 1 a 2 is the difference between the hydrogen bond acidity parameters of columns 1 and 2 b 1 b 2 is the difference between the hydrogen bond basicity parameters of columns 1. Inertsil ods 4 minimizes the dewetting phenomenon while maintaining the same retention characteristics to that of common ods columns. The original 2series inertsil phases, including silica, c4, c8, ods 2 and phenyl, created the high purity silica revolution in the late 1980s. Inertsil ods columns are general purpose, reversed phase c18 columns available in 5.

Inertsil odsp high steric selectivity l1 3, 5 10 450 inertsil odsep a polar functional group embedded l1 5 10 450 inertsil wp300 c18 analysis of high molecules l1 5 30 150 inertsil ods80a elute low molecule with a sharp peak shape l1 5 8 450 inertsil ods2 ultra pure silica gel is used l1 5 15 320 inertsil ods inertness 1st generation l1 5. Specifications of various inertsil ods columns surface area pore size purity carbon loading bondedphase structure endcapping inertsil ods4 450 m2g 100 a 99,999% 11 % monomeric inertsil ods3 450 m2g 100 a 99,999% 15 % monomeric inertsil odsp 450 m2g 100 a 99,999% 29 % polymeric no. The longawaited validated inertsil ods4v has now been added to our product lineup. Octadecylsilica ods is the industry standard packing material for hplc applications. Inertsil hplc column catalog inertsil hplc column catalog. The inertsil ods3 series represents a major advance in performance over the original inertsil 2series. Inertsil wp300 300a series wp300c18, c8, c4, diol, sil inertsil odshl 100a series. Strong retentivity, lower column backpressure, very inert. A 100% water mobile phase hplcpda analysis of melamine and related analogues. Phenomenex is a global manufacturer of uhplc, hplc columns, gc columns, sample preparation products and chromatography accessories and consumables. Figure 11 shows the application of this column to the analysis of cytochrome c tryptic digest. Reversed phase hplc columns comparison data on commonly used c18 phases analytical inc. Sd analytical, was established in the year of 2014.

Hplc columns c18 amide c4 c8 sec hilic phenyl t3 shield rp18. These columns are optimized for ultrafast hplc separations with more resolving power for small molecules. Therefore, inertsil ods4 can be used at ease under 100% aqueous eluents. As shown in figure 1, inertsil ods 3 has a relatively strong retentivity compared to commercially available ods columns. A 100% water mobile phase hplc pda analysis of melamine and related analogues. Inertsil 3series phases are based on a purer, higher surface area silica which is specially manufactured to provide maximum bonded phase coverage. Inertsil ods 3 has a relatively strong retentivity compared to commercially available ods columns. Reversedphase c18 columns ods versatile hybrid silica based hplc column ymctriart c18 ymctriart c18 is a hybrid silica based ods column emphasizing durability, low operating pressure, and excellent performance. Inertsil ods3 and ods3v capital hplc limited hplc columns. Inertsil ods 3 is still gl sciences most popular phase and continues to be used widely and reliably for long established methods in pharmaceutical, and contract research labs. Inertsil hplc columns for reversed phase separation 2. Hplc column technical guide introduction specifications shipping solvent mobile phase.

Ods columns are used for reversephase chromatography. This series features a variety of chemical bond bases such as c18,c8,nh2,cn, phenyl, hilic, and amide, and is able to meet a wide array of. Hplc column comparison screening study for reversed. Agilent technologies zorbax sbc18, waters symmetry c18, sigmaaldrich biotechnology supelcosil abz and gl sciences inertsil ods 2. Phenomenex uhplc, hplc, spe, gc leader in analytical. In general, elution time of inertsil ods 4 is shorter than inertsustain c18 and ods 3. Hplc columns hplc column selectivity measurements of more. Lc columns and consumables ed kim application engineer february, 2008. Inertsil ods 4 column name functional group inertsustain c18 inherits the advantages of all the current inertsil hplc columns, but now can be used for wide ph analysis with consistent performance. Inertsil diol enable sugars analysis with a long column. Therefore, inertsil ods 4 can be used at ease under 100% aqueous eluents. Inertsil ods sprint operates at low back pressures, even at high ow rates and shows excellent robustness and reproducibility. Inertsil ods 4, ods 3, ods sp, ods 2 reversedphase methanolwater columns 5. The original 2series inertsil phases, including silica, c4, c8, ods2 and phenyl, created the.

Inertsil ods3v series product data 1020 gl sciences. Furthermore, the packing material that achieves a stable analysis using 100% aqueous eluents provides another. Cortecs columns solidcore particle technology in 1. Inertsil ods sprint inertsil peptide c18 the inertsil peptide c18 column is speci cally suited to the analysis of peptide digests. Inertsil ods4, ods3, odssp, ods2 reversedphase methanolwater columns 5. Inertsil ods 5 100 350 14 yes no inertsil ods3 5 100 450 15 yes yes inertsil ods2 5 150 320 18. View all luna hplc column part numbers luna c18 2 is a recommended alternative to. A 100% water mobile phase hplcpda analysis of meamine. Hplc columns c18 amide c4 c8 sec hilic phenyl t3 shield.

Hplc column comparison screening study for reversed phase columns. Inertsil ods p high steric selectivity l1 3, 5 10 450 inertsil ods ep a polar functional group embedded l1 5 10 450 inertsil wp300 c18 analysis of high molecules l1 5 30 150 inertsil ods 80a elute low molecule with a sharp peak shape l1 5 8 450 inertsil ods 2 ultra pure silica gel is used l1 5 15 320 inertsil ods inertness 1st generation l1 5. Ods columns find a wide range of applications due to their high theoretical plate number and rapid equilibration. Figure 11 shows the application of this column to the analysis of cytochrome c tryptic. Comparison guide to c18 reversed phase hplc columns. Specifications of various inertsil ods columns surface area pore size purity carbon loading bondedphase structure endcapping inertsil ods 4 450 m2g 100 a 99,999% 11 % monomeric inertsil ods 3 450 m2g 100 a 99,999% 15 % monomeric inertsil ods p 450 m2g 100 a 99,999% 29 % polymeric no. Inertsil ods 4 a musthave column in your laboratory reversed phase ods and c8 columns comprise the vast majority of columns used in the hplc separation industry. Hypersil stationary phases are recognized as an industry standard in hplc. In general, elution time of inertsil ods4 is shorter than inertsustain c18 and ods3. Inertsil ods3 users can, therefore, readily change to the new, validated inertsil ods3v columns with complete confidence. We recommend inertsustain c18 or inertsil ods4 columns for all new method development.

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